To retain their usefulness, cultures that manufacture economically valuable products must be maintained in ways
that insure they will continue to be able to synthesize those valuable metabolites. Accordingly, they must be uncontaminated,
viable, and genetically stable. Maintaining Cultures for Biotechnology and Industry gives practical advice
necessary to preserve and maintain cells and microorganisms important to the biotechnology and pharmaceutical industries,
not just those strains currently being used but those yet to be discovered and engineered. This book is essential
for anyone working with cultures who wants to avoid the frustration of losing strains and needs to be able to devise
and evaluate new strategies for preservation.
Written by hands-on experts in their respective fields
Contains helpful tables and protocols for preserving or maintaining cultures of
Algae
Eubacteria
Actinomycetes
Fungi
Protozoa
Animal cells
Viruses
Plant germplasm
Plant viruses and viroids
Provides means to preserve cells by
Freezing
Lyophilizing
Drying
Cryoprotecting
Storing Spores
Continuously propagating and sub-culturing when absolutely necessary
Provides information needed to test cultures for stable retention of important characteristics
Gives principles needed to devise and evaluate preservation strategies for newly identified and newly engineered
cells and organisms
Lists culture repositories for each class of organism
Includes information for characterizing and monitoring recombinant organisms, especially important because
of their propensity for genetic instability
Discusses the history of the continually evolving field of culture preservation
Examines the importance of genetically stable cultures as it relates to maintaining patent positions
