This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic
source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the
major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis,
Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most
highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently
consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in
print), the series contains much material still relevant today--truly an essential publication for researchers
in all fields of life sciences.